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EM02-010

Extractime DNA Bacteria kit, 10 preps

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For purification of high quality bacterial gDNA
Available for risk-free testing.

The EXTRACTME DNA BACTERIA KIT is designed for a rapid and efficient purification of high quality bacterial gDNA from broth and plate cultures as well as frozen cells. The isolation protocol and buffer formulations were optimized for high isolation efficiency and DNA purity.

PRINCIPLE

DNA purification procedure consists of five steps and utilizes spin minicolumns with membranes which efficiently and selectively bind nucleic acids. During the first step, cell walls, membranes and proteins are degraded by lysis buffer and Proteinase K. To obtain an RNA-free DNA sample, RNA is removed by RNase A. After addition of chaotropic salts, lysate is applied to purification column membrane and DNA is bound. A two-step washing stage effectively removes impurities and enzyme inhibitors. Purified DNA is eluted with the use of a low ionic strength buffer (Elution Buffer) or water (pH 7.0–9.0) and may be used directly in all downstream applications such as PCR, qPCR, Southern blotting, DNA sequencing, enzymatic restriction, DNA ligation and so forth, or stored until ready to use.

SAMPLE MATERIAL: Broth or plate bacterial culture, frozen cell pellet
BINDING CAPACITY: approx. 40 μg DNA

EFFICIENCY:

  • up to 5 x 108 cells → 100%
  • 109 ÷ 3 x 109 cells → 75-90% (when the modified protocol for isolation from a great number of cells is followed)
  • 109 ÷ 3x 109 cells → ≤ 60% (when the standard isolation protocol is followed)
TIME REQUIRED: Approx. 40–60 minutes (including incubation time)
DNA PURITY: A260/A280 ratio = 1.7 – 1.9
More info on manufacturer's site
PROTOCOL

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